The help file for this program is available here
Enter the name of gene to delete
Enter desired length of target sequence
(i.e. length of primer excluding plasmid specific sequence)
The reverse primer is designed to allow controlled expression or N-terminal tagging of
full length proteins. Five forward primer sequences will be suggested to you. The first
primer is directly upstream of the stop codon. The remaining forward primers are
positioned away from the ORF in user-defined increments.
How far away (base pairs from ORF) would you like each subsequent primer to be?
Which N-Terminal tag would you like to use?
Long strings of identical bases are highlighted in colour.