The help file for this program is available here
Enter the name of gene to delete
Enter desired length of primer target sequence
Which plasmid will you use as a PCR template?
The forward primer is designed to allow C-terminal tagging of full length proteins.
Five reverse primers sequences will be suggested to you. The first primer is directly
downstream of the stop codon. The remaining reverse primers are positioned away from the
ORF in user-defined increments.
How far away (base pairs from ORF) would you like each subsequent primer to be?
Long strings of identical bases are highlighted in colour.